TCSPC Life Time (PICOQUANT)
Fluorescence Life time Microscope
MicroTime 100
The time-resolved microscope MicroTime 100 contains the complete optics and electronics for recording fluorescence decays in small volumes by means of Time-Correlated Single Photon Counting (TCSPC).
The system is based on a conventional upright microscope body. With the MicroTime 100, decay times down to some picoseconds can be resolved.
The system allows operation at laser repetition rates as high as 84 MHz and count rates up to several million counts/sec.
A laser coupling module with sophisticated beam shaping and focusing optics allows the use of external pulsed diode lasers. The system is designed to be used with the picosecond diode lasers of the LDH Series. Standard dichroic fi lter blocks are utilized to guide the light onto the sample and to fi lter out the fl uorescence light. Standard PMT modules or SPAD detectors can be provided in a single or dual channel detector confi guration.
All data acquisition as well as analysis functions of the MicroTime 100 are controlled by the SymPhoTime software.
Further available is an inverse microscope, the MicroTime 200, with up to 4 detection channels for simultaneous detection of either polarization anisotropy or multicolor experiments.
Features
- Complete microscope with laser coupling module
- Laser wavelengths from 375 nm to 810 nm
- Multiple detector options
- Upright microscope base
- XY-scanning Piezo stage for 2D-lifetime imaging
- Wide range scanner with a scan range up to several cm (on special request)
Key Applications
- Lifetime mapping
- Time-resolved microscopy in biology and chemistry
- Semiconductor wafer testing and analysis
- Materials research
- Cell biology and Fluorescence Lifetime Imaging (FLIM)
- Fluorescence Correlation Spectroscopy (FCS)
Key Specifications
| Excitation Sources | Picosecond diode laser wavelengths | 375 - 900 nm | |||
| Repetition rate | up to 40 MHz, (optional 80 MHz) | ||||
| Detectors | Type | PMT (PMA Series) | SPAD (PDM Series) | SPAD (SPCM-AQRH) | |
| Spectral range | 185 - 700 nm | 185 - 820 nm | 400 - 1000 nm | 400 - 1000 nm | |
| Dark counts (at 20 °C, typ. value) | < 50 cps | < 900 cps | < 250 cps | < 100 cps | |
| Instrument Response Function | typ. 200 ps | typ. 200 ps | typ. 50 ps | typ. 200 ps | |
| Data Acquisition | Type | PicoHarp 300 | TimeHarp 260 PICO | TimeHarp 260 NANO |
|
| Time resolution (bin width) | 4 ps | 25 ps | 1 ns | ||
| Dead time | < 95 ns | < 25 ns | < 1 ns | ||
| Time channels per curve | up to 65536 | 32768 | 32768 | ||
| Scanning (optional) | Type | high precision | |||
| Range | 80 × 80 µm | ||||
| Positioning accuracy | < 10 nm | ||||
| Operation & Electrical | PC requirements | Quad-core CPU > 3 GHz, RAM >= 4 GB, Windows™ 7/8 | |||
| Power requirements | 220/240 or 110/120 VAC, 50/60 Hz | ||||
| Dimensions | Microscope unit | 320 × 600 × 600 mm (w × d × h) | |||
